Of note, two RIF-R strains carrying a rare resistance-associated mutation, S450F, encoded by a double substitution TCG450TTC (results confirmed by Sanger sequencing, data not shown), were reported by Deeplex Myc-TB to carry two distinct single mutations, TCG450TTG (99.6%) coding for the Rif-R S450L substitution, and the synonymous TCG450TCC (S450S) substitution (98.8%). Here, MYC is linked to tuberculosis.