Although our IHC approach revealed that CD163 and MARCO expression in pancreatic cancer significantly increases and high CD163 and MARCO expression is correlated with poor prognosis, a more detailed understanding of MARCO+ macrophages is required, which can be obtained using more experimental methods such as Sc-RNA-seq, RNA-seq, and multiplex immunofluorescence. The gene discussed is CD163; the disease is pancreatic neoplasm.