Luciferase reporter assays showed that miR-124 is the direct target of MALAT1, and there was a potential negative correlation between miR-124 and MALAT1. shMALAT1 can suppress the proliferation, colony formation, and apoptosis of NSCLC cells, while miR-124 inhibitors can reverse this effect. This evidence concerns the gene MALAT1 and non-small cell lung carcinoma.