The expression of MALAT1 was negatively correlated with the expression of miR374b-5p and positively correlated with the expression of SRSF7. MiR-374b-5p is the target of MALAT1. Knockout of MALAT1 and miR-374b-5p overexpression can inhibit the proliferation, migration, and invasion of NSCLC cells and induce apoptosis. The gene discussed is SRSF7; the disease is non-small cell lung carcinoma.