Eventually, we determined 10 hub genes (Down-regulated genes: MYC, TGFB3, ADAMTS1, THBS1, RASD1, PCDH20; Up-regulated genes: MAMDC4, CYP7A1, GINS2, and PDLIM3) related to NAS and steatosis. Here, TGFB3 is linked to neonatal abstinence syndrome.