In the present study we used a BALB-c mouse model to characterize, in detail, the CD8+ T-cell responses to SAM-H1 candidate vaccine formulated with a cationic nanoemulsion (SAM-H1/CNE) and compared it with the response elicited by an MF59-adjuvanted monovalent influenza vaccine (aMIV). The gene discussed is CD8A; the disease is influenza.