To determine the function of the PMEPA1 in BLCA, we applied the Limma package (version: 3.40.2) of R software to study the DEGs between low- and high-PMEPA1 expression tissue in TCGA and 125 up-regulated DEGs and 804 down-regulated DEGs were found (Table S8 and Figures 2A, B). The gene discussed is PMEPA1; the disease is bladder transitional cell carcinoma.