HCC cells from 5 to 6 patients and the primary hepatocytes were stained with CK-18, ALB, ARG-1 (biomarkers of hepatocyte and hepatocarcinoma cells) and AFP (biomarker of hepatocarcinoma cells), with cancer-associated fibroblasts (CAFs, from HCC sample) as the negative control. This evidence concerns the gene AFP and hepatocellular carcinoma.