We show that overexpression of CRBP-1 induces the intracellular levels of RA to activate RARs/RXRs that transcriptionally promotes the expression of WIF1, a new target gene, then inhibits wnt/β-catenin signaling pathway, thus suppresses the stemness of liver CSCs in HCC. This evidence concerns the gene RBP1 and hepatocellular carcinoma.