In order to study the potential role of KIF4A play in the progress of ESCC, we used lentiviral KIF4A short hairpin RNAs (shRNAs) stable knockdown the expression of KIF4A in KYSE150 and TE1 cells, and a scrambled shRNA was used as a negative control (NC). The gene discussed is KIF4A; the disease is esophageal squamous cell carcinoma.