We found that MCT injection combined with IR operation significantly increased mPAP and RVSP in rats, and CYP2J2 overexpression decreased mPAP and RVSP in rats with PAH with LIRI model, but this effect was inhibited by C26 (the selective inhibitor of CYP2J2). Here, CYP2J2 is linked to pulmonary arterial hypertension.