Mapping of the most commonly observed missense mutations in TP53 revealed the R273 DNA contact point residue in the DNA binding domain as the most frequently mutated hotspot (9.45% in all cancers, 22% in PCa; Fig. 1C; Supplementary Fig. 1), further confirming previous studies that demonstrated the R273-p53 residue as frequently altered [8, 18]. This evidence concerns the gene TP53 and cancer.