Although MRPS31 showed a positive association with RB1 at mRNA level (Fig. 1F), to further examine the relationship between MRPS31 and RB1 loss in the clinical outcome of HCC, we divided the HCC samples into RB1 wild type (n = 210) and RB1 deleted type (n = 166) groups, based on RB1-DCN (threshold of −0.2 for DCN loss). This evidence concerns the gene RB1 and hepatocellular carcinoma.