In this study, DACH1 suppressed the invasiveness and metastasis of breast cancer cells via decreasing the expression of MMP9. DACH1 could interact with p65 and c-Jun respectively, and the ChIP-ReChIP assay showed that DACH1-p65 and DACH1-c-Jun complex could be recruited to the NF-κB binding site and AP-1 binding site respectively. The gene discussed is RELA; the disease is breast cancer.