qRT-PCR analysis was conducted on the cells bound to the microchannels to detect mRNAs associated with prostatic tissues, androgen receptor (AR), and prostate-specific antigen (kallikrein-related peptidase 3 or KLK3), as well as prostate tumor-derived genes prostate cancer antigen 3 (PCA3) and PSMA (Figure 2a). The gene discussed is PCA3; the disease is prostate neoplasm.