To investigate whether the PDAC cell lines used in this experiment possess the ability to synthesize CD95 and CD95L mRNA, we performed semi-quantitative gene expression analyses in five different PDAC cell lines (A818-6, A818-4, Colo-357, Panc89 and PancTuI-luc) and a T-lymphocyte cell line, JFL.13.3 (positive control for CD95L), and a breast cancer cell line, MDA-MB-468 (negative control for CD95L), by means of a PerfectProbe® PCR assay. This evidence concerns the gene FAS and breast cancer.