In the 21 specific clusters, 6 immune cell clusters represented as CD326−/lowCD45+, and 5 tumor epithelial cell clusters expressed CD326+CD45−/low, while the clusters showed that CD326−/low CD45−/low were defined as “other cells” which maybe dedifferentiated tumor cells, fibroblast, endothelial cells or other cell types. The gene discussed is EPCAM; the disease is neoplasm.