Therefore, to further confirm the mechanism by which B4GALNT1 acts in MIBC, we analyzed the published scRNA-seq data of eight bladder cancer tissues samples, and found that the major upregulated DEGs of fibroblasts in the high- and low-B4GALNT1 expression groups were significantly enriched in the extracellular exosome and the ECM-related signaling pathways. This evidence concerns the gene B4GALNT1 and urinary bladder carcinoma.