Whole-genome (DNA) sequencing revealed that dMMR3 and dMMR5 had a loss of function mutation in MSH2 (p.Glu483*), and inactivating mutations in MLH1 (p.I219V) and MSH3 (p.W1111R), indicating that these PDOs were derived from patients with Lynch syndrome (Figure 2). This evidence concerns the gene MLH1 and Lynch syndrome.