Persistence of these mutations in CR was shown not to be associated with worse EFS and OS, which indicates that these mutations represent a pre-malignant state where the acquisition of additional mutations is needed for the development of AML, similar to what has been proposed for DTA mutations [52], and that the acquisition of NPM1 mutations is a later event in the formation of leukemia [63]. The gene discussed is NPM1; the disease is acute myeloid leukemia.