In hepatocellular carcinoma, a distinct phenotype of CD5(hi)CD24(−/+)CD27(hi/+)CD38(dim) Bregs displaying high levels of PD-1 expression has been observed to exert pro-tumorigenic effects and T-cell suppression through IL-10 production after activation of the PD-1/L1 pathway [101]. This evidence concerns the gene PDCD1 and hepatocellular carcinoma.