However, our analysis showed that gene expression of the Hippo Signalling pathway is upregulated in the TP53 WT group of TCGA astrocytoma, and PPI analysis showed that the YAP1 high group (which was also associated with significantly decreased OS) was enriched for YAP1–TEAD complexes (Supplementary File 4: Figure S3) that drive transcription and proliferation [65]. This evidence concerns the gene TP53 and astrocytoma (excluding glioblastoma).