As shown in Figure 4a, treatment of both HT29 and CT26 cells with 15 μg/mL of SeNps for 24 h or 48 h resulted in the release of the nuclear HMGB1, evident by the increased levels of the protein in the cytoplasm of the cancer cells and the corresponding decrease in the nucleus, an effect that for both cell lines appears to be more prominent at 24 h of treatment. Here, HMGB1 is linked to cancer.