As AMBRA1 affected CTNNB1 transcription via ALDH1B1, which is a crucial CSC marker of colorectal cancer, we next investigated whether AMBRA1 expression affects the mRNA levels of several CSC-related β-catenin target genes, including LGR5, HIF1a, ALDH1A3, CD24, and SOX4. Here, HIF1A is linked to colorectal cancer.