In the present report, we established a proximity ligation assay (PLA) to detect and quantify the co-localization of FZD6 with the major canonical co-receptor LRP6 as an early event of the well-documented Wnt/β-catenin signal transduction after Wnt10B stimulation in PC-3 prostate cancer cells. This evidence concerns the gene LRP6 and Familial prostate cancer.