To verify GPI-80 secretion in EVs, soluble GPI-80 (sGPI-80) was measured in the plasma of prostate cancer patients and in the conditioned medium of PC3 cells expressing GPI-80 using sandwich ELISA with two types of anti-GPI-80 mAb clone, 3H9 (IgG1, used for capture) and 4D4 (IgG2a, used for detection). Here, VNN2 is linked to prostate carcinoma.