To investigate if advanced DMEM is as available for prion-infected cell cultures as classic DMEM, we cultured 22L scrapie prion-infected mouse neuroblastoma N2a cells, termed N2aC24L1-3 cells [15], in advanced DMEM with 2% FBS and in classic DMEM with 10% FBS, and compared PrPSc levels in the cell lysates by Western blotting with 6D11 anti-PrP antibody, which recognizes residues 97–100 of mouse PrP [16]. Here, PRNP is linked to neuroblastoma.