To elucidate whether shelterin complex mutations can predispose to the development of pediatric cancer, we analysed whole exome sequencing data of two independent parent–child cohorts of pediatric cancer patients (TRIO-D, n = 158 [16]; TRIO-DD, n = 111) for rare germline variants (minor allele frequency (MAF) < 0.2%) in the shelterin complex genes (Supplementary Table S1). Here, TRIO is linked to cancer.