In order to investigate the decreased LEA lectin’s specificity in the lesioned hemisphere, we performed counterstaining for the microglia marker ionized calcium-binding adaptor molecule-1 (Iba-1) and the astrocytic marker glial fibrillary acidic protein (GFAP) as both cell types undergo massive activation during brain ischemia [43,44]. This evidence concerns the gene AIF1 and brain ischemia.