To understand the increased recruitment of mMDSC in tumors grown in mice with Shb conditionally deleted in EC, we isolated tumor-derived CD45+ cells, performed qPCR for a number of potentially relevant genes, and compared the results with those of HC-deleted Shb using Vav1-Cre as a control to exclude off-target effects of Cdh5-CreERt2 in HC. The gene discussed is SHB; the disease is neoplasm.