In two independent studies, two variants of CAR have been created: (1) the intracellular signaling molecule DAP12 was cross-linked with the anti-PSCA single-chain Ab fragment scFv(AM1), resulting in the increased cytotoxicity of the YT NK cell line against PSCA-positive tumor cells compared to CAR-NK-containing CD3ζ signaling domain [198]; (2) DAP12 was cross-linked to the extracellular domain of NKG2D [199]. This evidence concerns the gene TYROBP and neoplasm.