Overexpression of miR‐145 inhibited KLF4 protein expression, suggesting that KLF4 was negatively correlated by miR‐145. Additionally, mechanical stretch downregulated miR‐145 expression by activating the stretch pathway, and downregulation of miR‐145 further stimulated KLF4 expression, which induced AD‐VSMC differentiation. This evidence concerns the gene KLF4 and Alzheimer disease.