In HCC cells treated with HULC siRNA, the levels of LC3‐II, Atg5, and Atg7 decreased significantly, as well as the level of Atg12 shown by IF staining (Figures 1B and 2A), suggesting the reduction of Atg12 conjugation system (Atg12/Atg7/Atg5) and autophagy. This evidence concerns the gene ATG12 and hepatocellular carcinoma.