NKAIN1 and glioblastoma: Knowing the strong predictive power of the top five genes, we were interested in investigating the biological relevance of these genes based on gene expression across subtypes and GBM survival analysis.9,12 Sodium/Potassium transporting ATPase Interacting 1 (NKAIN1) is a membrane-bound protein that interacts with beta subunit of the Sodium/Potassium ATPase.15 Although the gene is not well studied in GBM, NKAIN1 has the highest expression in proneural subtype as compared to both classical and mesenchymal subtype with no difference in survival (Figure 1A).