To determine the γδ T cells expressing immune markers in LTB individuals with NDM, PDM, and DM comorbidities, we used multicolor flow cytometry to delineate the UNS and Mtb antigen-specific (PPD, WCL) stimulated frequencies expressing immune (GMCSF, PD-1, CD69) markers (Figure 4). Here, PDCD1 is linked to diabetes mellitus.