PRF1 and neonatal diabetes mellitus: To determine the γδ T cells expressing cytotoxic markers in LTB individuals with NDM, PDM, and DM comorbidities, we used multicolor flow cytometry to delineate the baseline (UNS) and Mtb antigen-specific (PPD, WCL) stimulated frequencies expressing cytotoxic (perforin (PFN), granzyme B (GZE B), and granulysin (GNLSN) markers) (Figure 3).