CD8A and HIV infectious disease: Two methods were utilized to ascertain antigen-specific CD8+ T cells in three subjects for TL10 and one subject for RF10 with chronic HIV infection: 1) HLA class I tetramers loaded with either the NAE or AE form of the epitope (‘resting’ CD8+ T cells); and 2) activation induced marker (AIM) expression after peptide stimulation (‘activated’ CD8+ T cells).