Given that loss of TSC1 or TSC2 is a common occurrence in kidney AMLs from TSC patients13, we next performed global gene expression analysis comparison by means of whole transcriptome RNA sequencing (RNA-seq) of TSC2+/−, TSC2+/+, and TSC2−/− iPSC-derived organoids generated by differentiation of Day-9 metanephric mesenchymal cell aggregates in three-dimensional culture conditions22. The gene discussed is TSC2; the disease is tuberous sclerosis.