Our RNAseq data showing that levels of PKD1 mRNA in TSC2−/− AML organoids were comparable to mRNA levels in TSC+/− and TSC+/+ kidney organoids, indicated that the cystogenic mechanisms are solely driven by the loss of TSC2. Cyst formation can also be associated with a subtype of AML called AML containing epithelial cysts (AMLECs) that can be surrounded by condensed subepithelial cambium-like AML cells47,48. This evidence concerns the gene TSC1 and cyst.