Among the genes upregulated in the TSC2−/− organoids were: MLANA, PMEL, GPNMB, CTSK, and ACTA2, with median expression fold change 121x-, 88x-, 34x-, 14x-, 9.5x, respectively, all of which are known to be highly expressed in the renal angiomyolipoma (Fig. 3a). This evidence concerns the gene PMEL and kidney angiomyolipoma.