By means of the co-immunoprecipitation assay we determined that p21CIP1 directly interacts with pro-CASP3 in TSC2−/− AML organoid xenografts, and that this interaction is abrogated by treatment with Rapa-Nps, resulting in accumulation of cleaved CASP3 (Fig. 7e). This evidence concerns the gene TRERF1 and acute myeloid leukemia.