LIMD1 and clear cell renal carcinoma: In addition, we validated this effect in the clear-cell renal cell carcinoma cell line RCC48, using RNAi depletion of LIMD1. Here we observed increased sensitivity of these shLIMD1 cells to PF-477736 treatment, which could be rescued upon the expression of an RNAi-resistant Flag-His-tagged LIMD1 indicating that this increased sensitivity is specific to LIMD1 loss (Fig. S1C).