TGFB1 and neoplasm: Although no significant difference was observed in the tumor volumes of Saos-2/MG-63-bearing mice (Fig. 1E) or cell proliferation (Fig. 1F), Saos-2 and MG-63 cells treated with TGF-β exhibited a strengthened tumorigenesis capability (Fig. 1G), indicating of the potential relationship between TGF-β and stem-associated transcription factors in osteosarcoma.