Among the upstream proteins identified as being differentially regulated with naïve HA and HA‐enema treatment compared to vehicle (DSS) treatment in the DSS colitis mice model, the epithelial adherent/tight junctional proteins (E‐cadherin, β‐catenin, occludin), and cell surface receptor of HA, CD44 were selected for protein validation. This evidence concerns the gene OCLN and colitis.