Additionally, the regulated inflammatory markers that were inhibited in IPA analysis were further validated by ELISA with protein lysates of control, vehicle (PBS), naïve HA, HA‐enema treated colitis tissue samples by U‐plex biomarker cytokines panel (mice) for cytokines (IL‐1β, IL‐6) (Meso Scale Discovery) (Figure S4, Supporting Information). This evidence concerns the gene IL1B and colitis.