TGFB1 and non-small cell lung carcinoma: A549 cells and H1299 NSCLC cells were treated with TGFβ1 for 24 h prior to lysis and immunoblotting, and we observed that in response to TGFβ1, ULK1 phosphorylation of S555 tripled in A549 cells (Figure 1A) and doubled in H1299 cells (Figure 1B).