In another test dataset with 125 ESCC patients, 10 genes were detected, and 6 of them were survival associated (CLEC6A, DPP4, HTR2A, RASA3, SH3KBP1, and XCR1) (Supplementary Figure 2). The gene discussed is SH3KBP1; the disease is esophageal squamous cell carcinoma.