As displayed in Figure 2A, six pathways were significantly upregulated in ICC, namely DNA repair, E2F targets, G2M checkpoint, mitotic spindle, MYC targets V1, and TGF-β signaling; while seven pathways were dramatically downregulated, namely bile acid metabolism, cholesterol homeostasis, coagulation, fatty acid metabolism, pancreas beta cells, peroxisome, and xenobiotic metabolism. This evidence concerns the gene TGFB1 and intrahepatic cholangiocarcinoma.