The IHC results revealed higher levels of CXCL1 and VCAM-1 expression in patients with a higher-grade osteosarcoma than in those with a lower-grade osteosarcoma; the levels of CXCL1 and VCAM-1 expression were reflected by the tumor stage (Figures 7A, –C). This evidence concerns the gene VCAM1 and osteosarcoma.