Expression level of immune-checkpoint-relevant transcripts CD274 (PD-L1), CTLA-4, HAVCR2 (TIM3), LAG3, PDCD1 (PD-1), and TIGIT (VSIG9) were significantly higher in H2AFZhigh HCC patients, indicating that the H2AFZhigh HCC patients may be sensitive to immune checkpoint blockades (ICBs). The gene discussed is TIGIT; the disease is hepatocellular carcinoma.