To explore the effect of H2AFZ overexpression on the biological behavior of HCC cells, we performed flow cytometry (FCM), 5-Ethynyl-2’-deoxyuridine (EdU) proliferation assay, transwell migration assay, and Matrigel-transwell invasion assay on NC/TP53-siRNA/TP53-siRNA+H2AFZ-OE Huh7 and PLC/PRF/5 cells, respectively. The gene discussed is H2AZ2; the disease is hepatocellular carcinoma.