At first, we used the data from TCGA to analyze the mRNA expression of 10 subtypes of NHE family in COAD relative to the adjacent cancer, as well as the clinicopathological related effects (since SLC9B1, SLC9C1, and C2 are only expressed in male testis, they are not within the scope of this study). The gene discussed is SLC9C1; the disease is cancer.