Different features listed in the table represent the number and percentage of each variant in relation to the BC group, among these features the predominant cancer stage was stage II (67.5%), node status was N0 (35.6%), cancer grade was grade II (71.1%), tumor size was ≥ 2 cm- 5 cm (56.5%), NPI was > 3.4–5.4 (74.3%), positive ER was (79.8%), positive PR was (75.5%), negative Her2/neu expression was (55.2%), negative metastasis was (85.3%) and left operated breast was (61.3%). The gene discussed is ESR1; the disease is cancer.