Subsequently, we divided the tumor into two parts, extracted the total RNA and protein or embedding in paraffin, and using RNAScope (Fig. 5J and Supplementary Fig. 1B), immunohistochemistry (Fig. 5K), qPCR (Fig. 5L), and western blot (Fig. 5M) to detect the expression KDM4A-AS1, AR, AR-V7, and AR-V567es in each group. This evidence concerns the gene KDM4A and neoplasm.