Finally, to determine whether optimal ex vivo stimulation through the TCR, CD28, and provision of IL-12 could rescue the survival and function of dysfunctional TCRTg101, CTVhi and CTVlo TCRTg101 were purified from livers of day-17 leukemia-bearing mice and restimulated ex vivo with anti-CD3 and anti-CD28 antibodies or with C1498.B7.1 cells along with IL-2 and IL-12 for 5 days. This evidence concerns the gene CD28 and leukemia.