According to the results, Nrp‐1 mediates TGF‐β1 to improve the stability of regulatory CD4+CD25+ T cells and maybe a possible therapeutic target with the ability to improve the CD4+CD25+ Tregs associated negative immunoregulation that is related to the TGF‐β1/Smads cell signaling during sepsis. The gene discussed is CD4; the disease is Sepsis.