To further examine the role of E2F1/miR‐107/CCND1 axis in glioma progression, A172 cells stably transfected with NC mimic, miR‐107 mimic, sh‐NC + oe‐NC, sh‐E2F1 + oe‐NC and sh‐E2F1 + oe‐CCND1 were injected into nude mice for in vivo validation. This evidence concerns the gene CCND1 and central nervous system cancer.