In contrast, insulin-stimulated Akt phosphorylation was strongly reduced in WAT, muscle, and liver (Fig 4C, S6B–S6D Fig), indicating the development of insulin resistance in peripheral tissues of TnTp38γ/δact mice (in line with the higher circulating levels of NEFAs and triglycerides in these mice; see Fig 3G). Here, INS is linked to Insulin resistance.